The Birmingham Assay Office revealed it has raised £5,000 for the University Hospitals Birmingham (UHB) Charity.
The fund will be used to support NHS workers across Queen Elizabeth, Heartlands, Good Hope and Solihull Hospitals, it was announced.
The money came from a rainbow mark that the assay office launched last July.
The mark was available to be applied to jewellery alongside the usual Birmingham Assay Office mark.
This cost members of the public and jewellery retailers and manufacturers an extra £1, 100% of which was added to the now £5,000 pot.
Doug Henry, CEO and assay master, said: “We developed an idea which enabled us say ‘thank you’ to the NHS and to raise much needed funds for our local NHS Charity.
“We’d like to thank everyone who supported the initiative and help us raise funds to support NHS staff throughout the pandemic.”
Charlotte Schofield, director of fundraising at UHB Charity, said: “Our hospitals have been the worst affected in the UK during the pandemic, and therefore our staff have faced more deaths than any other trust.
“Our staff have worked tirelessly through the pandemic, and as they face the second wave of COVID-19 they need our support more than ever.
“This incredible donation from the Birmingham Assay Office will allow us to support our frontline staff, patients, their families and our local communities so that we can really make the difference that is needed.”